Summer Training program for Health Cluster Sr. Students 2019

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Required task

Brief

1

Sample handling

The trainee will recognize through this task type of the samples that he will dealwith and how to prevent the risk of those specimens.

2

Type of PPE

The necessary to comply with PPE and wearthem before starting the laboratory work.

3

Fire and safety

The trainee must know the emergency exits and fire extinguishers and check thelocation and contents of the chemical spillkit and howit is used.

4

Waste Management

Identify types of biological waste and types of containers used and how to get rid ofthem properly

5

Daily cleaning maintenance

Take careto clean the workplace before and after laboratory work and return thechemicals and tools used in the right place

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Required task

Brief

Reagent and consumables needed

1

Sampling and extraction

1. The trainee will trains on the type of samples that will be processed and prepared to undergo DNA extraction
2. Use of different workstations depends on the nature of extraction whetherDNA or RNA or PCR.

Need for DNA extraction kit

2

Instrumentation

1. After the process of extraction, training is carried out on the process of qualitative and quantitative measurement to determine the quantities required for the samples to be tested and this is done using several devices such as:
   • Genova Nano and Tecan.
   • Quatus flourometer and qubit.
2. Production stage is done by PCR either by using Cycler PCR OR Quant studio

• No need for reagent
• Need for reagent and tubes from promega
• Need for reagents

Result analysis

Through this step the trainee can read the results by using gell electrophoresis and I bright

For gell electrophoresis need reagent

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Required task

Brief

Reagent and consumables needed

1

• Preparation of bacterial growth media
• Sampling and streaking of bacteria from clinical source and surrounding environment
• Culturing bacteria into an agar plates.
• Gram staining
• Antibiotic sensitivity testing
• Using Biomic V3 for bacterial identification

By the end of this training, trainee will be able to:

• aseptically prepare bacterial media,
• Isolate single bacterial colonies
• Differentiate bacteria into Gram positive and negative
• Automatically identify bacteria up to the species level
• Evaluate the antimicrobial activity of chemotherapeutic agent

1. Nutrient agar Petri dishes
2. antibiotic disks
3. Loops
4. Cotton Swaps
5. Two types of  bacteria (Gram positive and negative)
6. Glass slides
7. Gram stain
8. Nutrient, blood and MacConkey agar plates
9. Kit for gram positive and gram negative identification

2

Instrumentation

• Biomic V3
• Light microscope
• Bacti- Cinerator
• Nephelometer
• Incubator
• Autoclave

• Automatic identification of bacteria
• Visualization of bacteria
• Instant sterilization of loops
• To measure concentration of bacteria
• For growth of bacteria
• For media sterilization

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Required task

Brief

Reagent and consumables needed

1

Training on basics of cell culture

By the end of this training, trainee will be able to :

• Apply basic aseptic techniques for cell culture.
• Evaluate the viability of the cultured cells.
• Use brightfield and fluorescent microscope.

• Cell lines and suitable media.
• Trypsin
• Serological pipette (10, 25ml)
• 15 ml tubes
• Petri dishes
• Culture flasks
• Live/dead stain
• PBS
• Ethanol

2

Training on culture and experimentation of chick embryo

By the end of this training, trainee will be able to :

• Understand the importance of chick embryo as a model in studying metabolic disorders such as heart disease and diabetes.
• Recognize the major developmental steps in chick embryo focusing on cardiovascular system, and resemblances to human.
• Doppler echocardiography technique to evaluate cardiac physiology.
• Perform chick embryo heart isolating techniques.

• Eggs
• Surgical tools
• Tyrode
• PBS
• Ethanol
• Transfer pipettes

3

Training on culture and experimentation of zebrafish embryo

By the end of this training, trainee will be able to :

• Understand the importance of zebrafish as a model in studying effects of toxic agents on organisms
• Learn the details of maintaining a zebrafish facility.
• Learn basic steps of breeding adult fish to generate embryos for experimentation.
• Recognize the major developmental steps in zebrafish embryo focusing on cardiovascular system.
• Learn how to use a stereo microscope for imaging small animals.
• Learn automated zebrafish software for cardiac assessment.

• Zebrafish embryo
• Imaging slides
• Petri dishes
• Egg water
• PBS
• Ethanol
• Transfer pipets

4

Training on RNA/ protein extraction

By the end of this training, trainee will be able to :

• Understand the functions of RNA and protein in the body.
• Extract RNA / protein  from samples using extraction kit
• Gain basic training in measuring RNA or Protein purity.

RNA/ protein  extraction kit

5

Training on QPCR

By the end of this training, trainee will be able to :

• Define the principals of QPCR
• Recognize the steps of how to run QPCR

( PCR kits , affinity script )
PCR plates /tubes
Specific Primers

6

Training on Western blot

By the end of this training, trainee will be able to :

• Define the principals the principal of wester blotting
• Extract of protein form cells or tissues

-Protein extraction kits.
-Chemical for the preparation of western blot buffers 
-Western blot instruments
-Specific Antibodies 

7

Training on basics of Microscopy

By the end of this training, trainee will be able to:

• Understand the basics of microscopy, components of a light and a fluorescent microscope
• Use image acquisition software to take images for static structures and image sequences for dynamic structures like a beating heart
• Use image processing software to make analysis on gathered images, like size measurements.

-Cells
-Antibodies
-Stains
-Coverslips
-Slides
-Mounting medium